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Episodis TV episodes i watched over the years. Use the HTML below. You must be a registered user to use the IMDb rating plugin. Photos Add Image Add an image Do you have any images for this title? Himself Megan Seely A Nature Research Journal. Humans are a visual-centric species, so it is no surprise that this partiality extends into the realm of science. Scientists' desire to see ever smaller details helped drive the development of sophisticated microscopes, but their utility for biologists was always constrained by the amount of contrast that could be generated between biological features.
Fluorescence provides a powerful means of overcoming the contrast problem in biology, and this power has resulted in an explosion in fluorescence imaging applications, to which we dedicate a Focus this month. Rather than relying on reflected or transmitted light to produce contrast, a stimulated fluorescent molecule emits light, allowing unprecedented contrast at microscopic scales and even allowing observations inside biological specimens.
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By using appropriate fluorescent molecules it is possible to track individual molecules in living systems or probe physiological changes within cells and tissue. Until recently, however, the introduction of such molecules was difficult and invasive. The development of membrane-permeable dyes and the use of fluorescent proteins now allow fluorophores to be introduced more easily and with less perturbation. Combined with powerful imaging modalities such as confocal, multiphoton and fiber-optic methods, these fluorophores allow deeper and more detailed imaging in living tissues and whole organisms.
The power of fluorescence imaging techniques has stimulated their use in biology to the point that they have become an integral part of the basic toolbox of many biologists. The proliferation of fluorescence imaging studies and the relative democratization of access to specialized instrumentation, however, should not obscure the fact that the quality of fluorescence images is affected by many interrelated variables. Variables such as light excitation, fluorescence emission, bleaching, spectral crosstalk and the physical characteristics of imaging equipment all dictate the quality of data that can be obtained in an experiment.
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All these phenomena are understood and well described at the level of the underlying physics, allowing the expert microscopist to optimize experimental design and techniques of observation. Although much of this basic physics is taught to all biology undergraduates, it is rarely taught with real-world examples that aspiring biologists will appreciate. As a result, many young biologists do not realize how their work relates to these underlying principles and therefore may not anticipate likely problems or know how to best overcome them.
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Signal strength and bleaching, for example, are probably the biggest problems encountered in fluorescence imaging experiments. No Thanks. Subscribe Subscribe. Subscribe to Weekly Story. Ask the Rabbi. Chabad Locator Find. Tools Directory of Tools:. Birthday Lookup. Candle Lighting Times. Chabad Locator. Date Converter.
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